Inhibition of ALA dehydratase activity in heme biosynthesis reduces cytoglobin expression which is related to the proliferation and viability of keloid fibroblasts

The aim of this study was to analyze the effect heme synthesis inhibition on cytoglobin expression and its correlation with keloid fibroblast viability proliferation. conducted primary culture fibroblasts. Heme in fibroblasts inhibited using succinyl acetone. We measured ALAD enzyme activity a colorimetric method; mRNA qRT-PCR; protein ELISA immunocytochemistry; MTT test; proliferation BrdU test. results showed that level lower treated SA (1, 2.5, 5 mM) than control. CYGB expressions were significantly cultured 2.5 mM control 1 SA. decreased when concentration increased. In conclusion, use acetone at 1; 2.5; caused decrease which indicated synthesis. Inhibition can affect expression, correlates